We undertook a
genome-wide search for novel noncoding
RNAs (nc
RNA) in the malaria parasite
Plasmodium falciparum. We used the
RNAz program to predict structures in the noncoding regions of the
P. falciparum 3D7
genome that were conserved with at least one of seven other
Plasmodium spp.
genome sequences. By using Northern blot analysis for 76 high-scoring predictions and microarray analysis for the majority of candidates, we have verified the expression of 33 novel nc
RNA transcripts including four members of a nc
RNA family in the asexual blood stage. These transcripts represent novel structured nc
RNAs in
P. falciparum and are not represented in any
RNA databases. We provide supporting evidence for purifying selection acting on the experimentally verified nc
RNAs by comparing the nucleotide substitutions in the predicted nc
RNA candidate structures in
P. falciparum with the closely related chimp malaria parasite
P. reichenowi. The high confirmation rate within a single parasite life cycle stage suggests that many more of the predictions may be expressed in other stages of the organism’s life cycle.
Source - http://genome.cshlp.org/content/18/2/281.abstract?sid=40206306-7c2e-4b18-96b3-8319fa594a4b
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