Small regulatory
RNAs have recently emerged as key regulators of eukaryotic gene expression. Here we used high-throughput sequencing to determine small
RNA populations in the germline and soma of the African clawed frog
Xenopus tropicalis. We identified a number of mi
RNAs that were expressed in the female germline. mi
RNA expression profiling revealed that miR-202-5p is an oocyte-enriched mi
RNA. We identified two novel mi
RNAs that were expressed in the soma. In addition, we sequenced large numbers of Piwi-associated
RNAs (pi
RNAs) and other endogenous small
RNAs, likely representing endogenous si
RNAs (endo-si
RNAs). Of these, only pi
RNAs were restricted to the germline, suggesting that endo-si
RNAs are an abundant class of small
RNAs in the vertebrate soma. In the germline, both endogenous small
RNAs and pi
RNAs mapped to many high copy number loci. Furthermore, endogenous small
RNAs mapped to the same specific subsets of repetitive elements in both the soma and the germline, suggesting that these
RNAs might act to silence repetitive elements in both compartments. Data presented here suggest a conserved role for mi
RNAs in the vertebrate germline. Furthermore, this study provides a basis for the functional analysis of small regulatory
RNAs in an important vertebrate model system.
Source - http://genome.cshlp.org/content/19/10/1766.abstract?sid=4be18750-d5af-4896-b05e-eeb49c944425
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